The technical features of PCR laboratory products mainly include the following aspects:
Strong specificity: PCR technology has a high degree of specificity and can accurately amplify specific DNA sequences without producing non-specific amplification products.
High sensitivity: PCR technology has a very high sensitivity and can detect the target DNA sequence from a very small amount of starting template. For example, one target cell can be detected from 1 million cells, or the presence of 3 bacteria can be detected.
High amplification efficiency: During the PCR reaction, the number of DNA copies increases exponentially, and a large number of DNA copies can be generated in a short time.
Easy and fast operation: PCR technology is simple and fast to operate, suitable for large-scale gene amplification needs, and combined with sequencing technology, it can quickly identify mutant alleles in DNA samples.
Applicable to a variety of application scenarios: PCR technology is applicable to a variety of application scenarios, including specific amplification of target DNA sequences from a small amount of starting material, detection of specific sequences in complex mixtures, cloning alternative methods, etc.
The basic principle of PCR technology is to rapidly enzymatically amplify specific gene (DNA) fragments in vitro of animals and plants through a pair of primers. After multiple thermal cycles, the number of specific genes contained in the amplified product is exponentially greater than the number of original templates, making it p