Real-time Fluorescence Quantitative PCR Analyzer

Your Professional Real-time Fluorescence Quantitative PCR Analyzer Supplier!

 

Kleibertus(Haikou) Electronic Technology GmbH is a foreign trade factory focusing on the life and health industry, committed to independent research and development, production and sales of high-quality products covering the entire medical and health industry chain. Our products cover areas such as medical diagnosis, in vitro diagnostic equipment, biosafety protection, disinfection and sterilization, purification engineering, cold chain transportation and medical beauty products, truly realizing a one-stop solution.

 

 
 
Why Choose Us
 
 
 

Full Industry Chain Coverage

Our products cover medical diagnosis, in vitro diagnostic equipment, biosafety protection, disinfection and sterilization, purification engineering, cold chain transportation and medical beauty products, truly realizing a one-stop solution.

 
 

Authoritative Certification Guarantee

The products have passed international certifications such as EU CE, EN, US NSF, FDA, etc., and the quality standards strictly meet the needs of the global market, winning high recognition from international customers.

 
 

Global Layout

Our business covers more than 190 countries and regions around the world. With an efficient international supply chain and customer service network, we ensure a quick response to global customer needs.

 
 

Leading in Technology and R&D

We always adhere to technology-driven, continue to increase R&D investment, ensure that our products always maintain high quality and high performance, and can meet the diversified needs of different markets.

 

 

Our Best-Selling Real-time Fluorescence Quantitative PCR Analyzer

 

Our Company Has More Products to Offer, Please Browse the Product Categories on the Website, If You Have Any Needs, Please Contact Us.

 
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Fluorescent Quantitative PCR Detection Machine

The Fluorescent Quantitative PCR Detection Machine is a highly advanced system designed to provide sensitive, specific detection and quantification of nucleic acid targets. It features state-of-the-art six-level independent temperature control technology, ensuring precise and consistent temperature regulation for accurate and repeatable results.

 
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Fluorescence Quantitative PCR Detections

The Fluorescence Quantitative PCR Detection system is engineered for high-efficiency, precise nucleic acid analysis, incorporating state-of-the-art scanning technology. It offers both full scan and formula line scan modes, with an impressive 5.5-second 96-well dual-color scan that significantly accelerates your workflow.

 
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Fluorescence Quantitative PCR Detection System

This Fluorescence Quantitative PCR Detection System is a high-performance solution designed for sensitive and specific nucleic acid detection and quantification. With its powerful detection algorithms, optimized qPCR reagents, and intuitive software, it unlocks the full potential of qPCR technology, supporting a wide array of applications including infectious disease research, food pathogen detection, waterborne pathogen testing, stem cell studies, oncology, genetic disease research, and agricultural biotechnology.

Fluorescent Quantitative PCR Detection Machine

 

 

Real-time Fluorescence Quantitative PCR Analyzer Technical Parameters

 

 

Product Name

Fluorescence Quantitative PCR Detection System

Model

MA-6000

Sample Capacity

96x0.2ml PCR plate, 12x8-strip tubes, 96*0.2ml single tube

Reaction System

6~125μl

Dynamics Range

1-1010 copies

Channel

5

Emission Light

Full-wavelength maintenance-free halogen tungsten lamp

Detector

-20℃ CCD

Detection Path

F1

F2

F3

F4

F5

Suitable Probe/Dye

FAM/SYBR Green/Eva Green/LC Green/Fluorescein

VIC/HEX/TET/Cy3/JOE/Alex a555

ROX/Cy3.5/Texas Red

Cy5/LC Red640

Cy5.5/LC Red705

Excitation Wavelength

380~780nm

Detection Wavelength

380~780nm

Fluorescence Detection Repeatability

CV≤3%

Fluorescence Detection Precision

CV≤ 5%

Sample Detection Repeatability

CV≤3%

Fluorescence Detection Linearity

r≥0.980

Confidence

It can effectively distinguish 5000 and 1000copies with99.8% confidence degree

Module Temp. Range

4-100℃(resolution:0.1℃)

Heating Speed

5.0℃/s(Max), 3.5℃/ s(Avg)

Cooling Speed

5.0℃/s(Max), 3.2℃/ s(Avg)

Temp. Accuracy

±0.1℃

Temp. Uniformity

≤ ±0.25℃

Number of Temp. Control Areas

6

 

Gradient Temp. Range

1-32℃

Hot-Lid Temp. Range

104℃, Automatic Hot-lid

Special Function

Absolute quantification, Relative Quantification, Genotyping, Amplification efficiency calculation, Melting curve.

Operation System

Windows 7 64-bit or higher

Power Supply

100~240V,50~60Hz

Dimension(L*W*H) mm

600*390 *320

Port Method

USB Port

Packing Size(L*W*H) Mm

850*600 *650

Gross Weight

51

 

Pros of Real-Time Fluorescence Quantitative PCR Analyzer

High sensitivity: Real-time fluorescence quantitative PCR analyzer is a highly sensitive method for detecting nucleic acid sequences, with the ability to detect even a single copy of the target sequence.


Specificity: Real-time fluorescence quantitative PCR analyzer primers can be designed to be highly specific to the target sequence, reducing the likelihood of false-positive results.

Real-time monitoring

Real-time fluorescence quantitative PCR analyzer allows for real-time monitoring of the amplification process, providing rapid and accurate results without the need for gel electrophoresis.

High-throughput

Real-time fluorescence quantitative PCR analyzer can be performed in high-throughput formats, allowing for the analysis of large numbers of samples at once.

Versatile

Real-time fluorescence quantitative PCR analyzer can be used for a wide range of applications, including gene expression analysis, clinical diagnostics, pathogen detection, and food safety testing.

 

Fluorescence Markers Used in Real-Time Fluorescence Quantitative PCR Analyzer

 

 

productcate-905-481

 

Components of Real-Time Fluorescence Quantitative PCR Analyzer

Reaction module

Samples are heated and cooled to precise temperatures to promote nucleotide denaturation, annealing, and then polymerase-mediated extension for each round of DNA amplification

Optical detection system

In the presence of a fluorescent reporter,, measurement of the fluorescence intensity of each PCR reaction enables determination of the presence of a target of interest within an experimental sample

Instrument software

Real-time fluorescence quantitative PCR analyzer are typically controlled by an attached computer running specialized software that initiates and monitors runs and then facilitates interpretation of the results. Some instruments, include an integrated interface and can be run without a computer. A computer is still needed for data analysis

 

Fluorescence Quantitative PCR Detections

 

Applications of Real-time Fluorescence Quantitative PCR Analyzer

Gene expression analysis
Cancer research
Drug research
Disease diagnosis and management
Viral quantification
Food testing
GMO food
Animal and plant breeding
Gene copy number

 

The Real-Time Fluorescence Quantitative PCR Analyzer process in Detail

 

 

Denaturation: The double-stranded DNA is heated to about 94-98 °C (201-208 °F) to split it into single strands. This opens up access for the primer that will bind to the individual strands in the next step.


Annealing: At a temperature of 50-65 °C (122-149 °F), specific primers bind to the complementary regions on the single DNA strands. These primers serve as the starting point for the polymerase, which will synthesize the DNA in the next step.


Elongation: The temperature is increased to approx. 72 °C (162 °F) to promote taq polymerase activity. This enzyme elongates the DNA strand by binding free nucleotides to the primer and creating a new, complementary strand. Since the taq polymerase takes effect again in each new cycle, the DNA amount also doubles each cycle.

 

 

Packaging and Shipping

 

 

Packaging

Foam, plastic bags, cartons

Transportation methods

Road transport, rail transport, sea transport, air transport

 

 

Our Certificate

 

 

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Video

 

 

 

 

FAQ

 

 

Q: What are the advantages of real-time fluorescence quantitative PCR analyzer?

A: Less experimental variation since both reactions take place in the same tube.
Fewer pipetting steps reduces risk of contamination.
Suitable for high throughput amplification/screening.
Fast and highly reproducible.

Q: What Does real-time fluorescence quantitative PCR analyzerMeasure in Real Time?

A: Real-time fluorescence quantitative PCR analyzer measures the amount of RNA present in a sample by first converting it into complementary DNA (cDNA) using reverse transcriptase, followed by real-time PCR amplification. This technique is commonly used to quantify gene expression levels, as it allows for the measurement of mRNA levels in real time.

Q: What are the applications of real-time fluorescence quantitative PCR analyzer?

A: Quantify gene expression levels.
Validate RNAi to study loss of function of selective genes.
Detect pathogens such as viruses for the diagnosis of infectious diseases.
Detect genetically modified organisms (GMOs)

We're well-known as one of the leading real-time fluorescence quantitative PCR analyzer suppliers in China. Please feel free to buy high quality real-time fluorescence quantitative PCR analyzer in stock here from our factory. For customized service, contact us now.

Fluorescence Quantitative PCR Detections, Novel Coronavirus 2019 nCoV Nucleic Acid Detection Kits, Gene Amplification Instrument

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